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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MICA1 rabbit pAb
- 货号:YT6400
- 应用:WB;IHC
- 种属:Human;Mouse;Rat
- 免疫原:
- Synthesized peptide derived from human MICA1 AA range: 155-205
- 特异性:
- This antibody detects endogenous levels of MICA1 at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015],
- 功能:
- cofactor:FAD .,domain:The C-terminal coiled coil part contains the plexin-interacting region.,function:May be a cytoskeletal regulator that connects NEDD9 to intermediate filaments.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 1 LIM zinc-binding domain.,subunit:Associates with the SH3 domain of NEDD9. Interacts with VIM and PLXNA3. Interacts with RAB1B.,tissue specificity:Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells.,
- 细胞定位:
- Cytoplasm. Cytoplasm, cytoskeleton. Midbody . Accumulates transiently at the abscission site before abscission occurs. .
- 组织表达:
- Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells.
- Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).