• 靶点：
• RAD1

• 简介：
• >>Cellular senescence

• 基因名称：
• RAD1 REC1

• 蛋白名称：
• RAD1

• Human Gene Id：
• 5810

• Human Swiss Prot No：
• O60671

• 免疫原：
• Synthesized peptide derived from human RAD1

• 特异性：
• This antibody detects endogenous levels of human RAD1

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IHC 1:50-200, WB 1:500-2000. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• Cell cycle checkpoint protein RAD1 (hRAD1;EC 3.1.11.2;DNA repair exonuclease rad1 homolog;Rad1-like DNA damage checkpoint protein)

• 实测条带：
• 32kD

• 背景：
• This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009],

• 功能：
• catalytic activity:Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.,function:Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity

• 细胞定位：
• Nucleus .

• 组织表达：
• Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).

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