PI 3 kinase p85α Polyclonal Antibody
- 货号:YT6156
- 应用:IF;WB;IHC;ELISA
- 种属:Human;Mouse;Rat;Pig
- 靶点:
- PI3 Kinase P85α
- 简介:
- >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Phosphatidylinositol signaling system;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Axon guidance;>>VEGF signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>JAK-STAT signaling pathway;>>Natural killer cell mediated cytotoxicity;>>T cell receptor signaling pathway;>
- 基因名称:
- PIK3R1
- 蛋白名称:
- Phosphatidylinositol 3-kinase regulatory subunit alpha
- Human Gene Id:
- 5295
- Human Swiss Prot No:
- P27986
- Mouse Gene Id:
- 18708
- Mouse Swiss Prot No:
- P26450
- Rat Gene Id:
- 25513
- Rat Swiss Prot No:
- Q63787
- 免疫原:
- Synthesized peptide derived from PI 3-kinase p85α . at AA range: 550-630
- 特异性:
- This antibody detects endogenous levels of PI 3-kinase p85α.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PIK3R1;GRB1;Phosphatidylinositol 3-kinase regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha
- 实测条带:
- 54kD,83kD
- 背景:
- Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],
- 功能:
- disease:Defects in PIK3R1 are a cause of severe insulin resistance.,domain:The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.,function:Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.,PTM:Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.,similarity:Belongs to the PI3K p85 subunit family.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH2 domains.,subunit:Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylat
- 细胞定位:
- nucleus,cytoplasm,cis-Golgi network,cytosol,plasma membrane,cell-cell junction,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,membrane,perinuclear endoplasmic reticulum membrane,
- 组织表达:
- Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
Proteomic Study on the Reproductive Toxicity of Tripterygium Glycosides in Rats WB Rat testis tissue /
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An Integrated Approach Based on Network Pharmacology Combined with Experimental Verification Reveals AMPK/PI3K/Akt Signaling is an Important Way for the Anti-Type 2 Diabetic Activity of Silkworm Excrement. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy Diabet Metab Synd Ob. 2021 Feb;14:601-616 WB Human 1:1000 HepG2 cell
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Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats. MEDICAL SCIENCE MONITOR Med Sci Monitor. 2017; 23: 5599–5612 WB Rat 1: 400 liver tissue
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Effects of Agriophyllum squarrosum extracts on glucose metabolism in KKAy mice and the associated underlying mechanisms. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2019 Sep;241:112009 WB Mouse 1:1000 liver
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Long Non-coding RNA GAS5 Maintains Insulin Secretion by Regulating Multiple miRNAs in INS-1 832/13 Cells. Frontiers in Molecular Biosciences Front Mol Biosci. 2020; 7: 559267 WB Rat 1 : 1000 INS-1 832/13 cell
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货号:YT6156
Sattar, Hamid, et al. "Selenoprotein-U (SelU) knockdown triggers autophagy through PI3K–Akt–mTOR pathway inhibition in rooster Sertoli cells." Metallomics 10.7 (2018): 929-940.
货号:YT6156
Yang, Jie, et al. "Selenium deficiency-induced thioredoxin suppression and thioredoxin knock down disbalanced insulin responsiveness in chicken cardiomyocytes through PI3K/Akt pathway inhibition." Cellular signalling 38 (2017): 192-200.
货号:YT6156
Wang, Wei-wei, et al. "Telmisartan reduces atrial arrhythmia susceptibility through the regulation of RAS–ERK and PI3K–Akt–eNOS pathways in spontaneously hypertensive rats." Canadian journal of physiology and pharmacology 93.8 (2015): 657-665.
货号:YT6156
Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic
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5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s
货号:YT6156
Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic
货号:YT6156
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- Antibody-FAQs
- 产品图片
- Fan, Hua, et al. "Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats." Medical science monitor: international medical journal of experimental and clinical research 23 (2017): 5599.
- Western Blot analysis of various cells using primary antibody diluted at 1:500(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)