ImmunoWay Biotechnology Company |抗体|病理抗体|诊断抗体

Fibronectin Polyclonal Antibody

货号：YT5848
应用：WB;IHC;IF;ELISA
种属：Human;Rat;Mouse;

产品详情
文献 ( 2 )
操作手册

靶点：
Fibronectin

简介：
>>PI3K-Akt signaling pathway;>>Focal adhesion;>>ECM-receptor interaction;>>Regulation of actin cytoskeleton;>>AGE-RAGE signaling pathway in diabetic complications;>>Bacterial invasion of epithelial cells;>>Yersinia infection;>>Amoebiasis;>>Human papillomavirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Small cell lung cancer

基因名称：
FN1 FN

蛋白名称：
Fibronectin

Human Gene Id：
2335

Human Swiss Prot No：
P02751

Mouse Gene Id：
14268

Mouse Swiss Prot No：
P11276

免疫原：
Synthetic peptide from human protein at AA range: 2304-2375

特异性：
The antibody detects endogenous Fibronectin

组成：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源：
Polyclonal, Rabbit,IgG

稀释：
IHC 1:50-200, ELISA 1:10000-20000,WB 1:500-2000. IF 1:50-200

纯化工艺：
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

浓度：
1 mg/ml

储存：
-15°C to -25°C/1 year(Do not lower than -25°C)

其他名称：
Fibronectin (FN;Cold-insoluble globulin;CIG) [Cleaved into: Anastellin;Ugl-Y1;Ugl-Y2;Ugl-Y3]

分子量：
272kD

背景：
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016],

功能：
alternative products:Additional isoforms seem to exist,developmental stage:Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.,disease:Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2) [MIM:601894]; also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life.,function:Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth

细胞定位：
Secreted, extracellular space, extracellular matrix .

组织表达：
Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level) (PubMed:29777959). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine (PubMed:17614963).

The mechanism of mulberry leaves against renal tubular interstitial fibrosis through ERK1/2 signaling pathway was predicted by network pharmacology and validated in human tubular epithelial cells. PHYTOTHERAPY RESEARCH 2019 Jun 17 WB Human 1:600 HK-2 cell
货号：YT5848

Astragaloside IV inhibits palmitate-mediated oxidative stress and fibrosis in human glomerular mesangial cells via downregulation of CD36 expression. Pharmacological Reports Pharmacol Rep. 2019 Mar;71(2):319-329 WB Rat 1:2000 kidney
货号：YT5848