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Flk-1/VEGFR2 Polyclonal Antibody

货号：YT5845
应用：IF;WB;IHC;ELISA
种属：Human;

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靶点：
Flk-1/VEGFR2

简介：
>>EGFR tyrosine kinase inhibitor resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>PI3K-Akt signaling pathway;>>VEGF signaling pathway;>>Focal adhesion;>>Proteoglycans in cancer;>>Fluid shear stress and atherosclerosis

基因名称：
KDR FLK1 VEGFR2

蛋白名称：
VEGFR2

Human Gene Id：
3791

Human Swiss Prot No：
P35968

Mouse Swiss Prot No：
P35918

Rat Swiss Prot No：
O08775

免疫原：
Synthetic peptide from human protein at AA range: 1268-1341

特异性：
The antibody detects endogenous VEGFR2

组成：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源：
Polyclonal, Rabbit,IgG

稀释：
IF 1:50-200 WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. Not yet tested in other applications.

纯化工艺：
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

浓度：
1 mg/ml

储存：
-15°C to -25°C/1 year(Do not lower than -25°C)

其他名称：
Vascular endothelial growth factor receptor 2 (VEGFR-2;EC 2.7.10.1;Fetal liver kinase 1;FLK-1;Kinase insert domain receptor;KDR;Protein-tyrosine kinase receptor flk-1;CD antigen CD309)

分子量：
152kD

背景：
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009],

功能：
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 7 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Interacts with MYOF (By similarity). Interacts with SHB; upon VEGF activation. Interacts with HIV-1 Tat.,

细胞定位：
Cell junction . Endoplasmic reticulum . Cell membrane . Localized with RAP1A at cell-cell junctions (By similarity). Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610). .; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.; [Isoform 2]: Secreted .; [Isoform 3]: Secreted.

组织表达：
Detected in cornea (at protein level). Widely expressed.

Marine bromophenol bis(2,3-dibromo-4,5-dihydroxybenzyl) ether, represses angiogenesis in HUVEC cells and in zebrafish embryos via inhibiting the VEGF signal systems. BIOMEDICINE & PHARMACOTHERAPY 2015 Sep 07 WB Human HUVECs
货号：YT5845

Marine bromophenol bis (2, 3-dibromo-4, 5-dihydroxybenzyl) ether, represses angiogenesis in HUVEC cells and in zebrafish embryos via inhibiting the VEGF signal systems." Biomedicine & Pharmacotherapy 75 (2015): 58-66.
货号：YT5845

Li, Na, et al. "Asparaginyl endopeptidase may promote liver sinusoidal endothelial cell angiogenesis via PI3K/Akt pathway." Revista espanola de enfermedades digestivas: organo oficial de la Sociedad Espanola de Patologia Digestiva111 (2018).
货号：YT5845

Acute hypoxia promotes the liver angiogenesis of largemouth bass (Micropterus salmoides) by HIF - Dependent pathway FISH & SHELLFISH IMMUNOLOGY Liulan Zhao WB,IHC Fish
货号：YT5845

Tetrahedral framework nucleic acids inhibit pathological neovascularization and vaso-obliteration in ischaemic retinopathy via PI3K/AKT/mTOR signalling pathway CELL PROLIFERATION Xiaoyan Ding IF,WB Human human umbilical vein endothelial cells (HUVECs)
货号：YT5845

Gualou Guizhi decoction promotes therapeutic angiogenesis via the miR210/HIF/VEGF pathway in vivo and in vitro. Lihong Nan WB Rat,Human Ischemic brain tissue Human umbilical vein endothelial cell (HUVEC)
货号：YT5845

Kaempferol inhibited invasion and metastasis of gastric cancer cells by targeting AKT/GSK3β pathway based on network pharmacology and molecular docking JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH Xia-Qing Gao WB Human 1:1000 HGC27 cell,MKN45 cell
货号：YT5845