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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MUM1 Polyclonal Antibody
- 货号:YT5753
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse;Rat
- 免疫原:
- The antiserum was produced against synthesized peptide derived from the C-terminal region of human MUM1. AA range:661-710
- 特异性:
- MUM1 Polyclonal Antibody detects endogenous levels of MUM1
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- melanoma associated antigen (mutated) 1
- 背景:
- PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MUM1 family.,
- 功能:
- PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MUM1 family.,
- 细胞定位:
- Nucleus . Recruited to DNA damage sites via its interaction with the BRCT domain of TP53BP1.
- 组织表达:
- Brain,Melanoma,Placenta,PNS,Thyroid,
- Western Blot analysis of mouse-kidney mouse-heart mouse-brain cells using MUM1 Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).