• 靶点：
• Villin

• 基因名称：
• VIL1 VIL

• 蛋白名称：
• Villin

• Human Gene Id：
• 7429

• Human Swiss Prot No：
• P09327

• Mouse Gene Id：
• 22349

• Mouse Swiss Prot No：
• Q62468

• 免疫原：
• Synthesized peptide derived from Villin at AA range: 601-650

• 特异性：
• Villin Polyclonal Antibody detects endogenous levels of Villin

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500-2000, ELISA 1:10000-20000

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• villin 1

• 实测条带：
• 90kD

• 背景：
• This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008],

• 功能：
• domain:Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.,function:Ca(2+)-regulated actin-binding protein.,similarity:Belongs to the villin/gelsolin family.,similarity:Contains 1 HP (headpiece) domain.,similarity:Contains 6 gelsolin-like repeats.,subunit:Monomer.,tissue specificity:Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.,

• 细胞定位：
• Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip . Cell projection, filopodium . Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment. .

• 组织表达：
• Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level).

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