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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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SCGF Polyclonal Antibody
- 货号:YT5719
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- C-type lectin domain family 11 member A
- 免疫原:
- The antiserum was produced against synthesized peptide derived from the N-terminal region of human CLEC11A. AA range:1-50
- 特异性:
- SCGF Polyclonal Antibody detects endogenous levels of SCGF protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CLEC11A;CLECSF3;LSLCL;SCGF;C-type lectin domain family 11 member A;C-type lectin superfamily member 3;Lymphocyte secreted C-type lectin;Stem cell growth factor;p47
- 背景:
- This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined. [provided by RefSeq, Jul 2008],
- 功能:
- developmental stage:In the bone marrow, expression is limited to immature neutrophils. Expression was not detected in circulating mature neutrophils.,function:Stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. Acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. Suppresses SCF-stimulated erythrocyte proliferation.,online information:Stem cell growth factor,PTM:O-glycosylated. Probably sulfated on the O-glycans.,similarity:Contains 1 C-type lectin domain.,tissue specificity:Expressed in skeletal tissues including bone marrow, chondrocytes, primary ossification center-associated cells, the perichondrium and periosteum. Lower levels of expression were detected in spleen, thymus, appendix and fetal liver.,
- 细胞定位:
- Cytoplasm . Secreted .
- 组织表达:
- Expressed in skeletal tissues including bone marrow, chondrocytes, primary ossification center-associated cells, the perichondrium and periosteum. Lower levels of expression were detected in spleen, thymus, appendix and fetal liver.
![](/uploadfiles/PIC/yt5719wb4148142088.jpg)
- Western blot analysis of MOUSE-SPLEEN RAT-SPLEEN using CLEC11A antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
![](/uploadfiles/PIC/yt5719ihchuman-skin.jpg)
- Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100