NHERF-2 Polyclonal Antibody
- 货号：YT5676
- 应用：WB;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Aldosterone-regulated sodium reabsorption
- 蛋白名称：
- Na(+)/H(+) exchange regulatory cofactor NHE-RF2
- 免疫原：
- Synthesized peptide derived from NHERF-2 . at AA range: 20-100
- 特异性：
- NHERF-2 Polyclonal Antibody detects endogenous levels of NHERF-2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SLC9A3R2;NHERF2;Na(+)/H(+) exchange regulatory cofactor NHE-RF2;NHERF-2;NHE3 kinase A regulatory protein E3KARP;SRY-interacting protein 1;SIP-1;Sodium-hydrogen exchanger regulatory factor 2;Solute carrier family 9 isoform A3 regulatory factor 2;Tyrosine kinase activator protein 1;TKA-1
- 背景：
- This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],
- 功能：
- function:Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.,similarity:Contains 2 PDZ (DHR) domains.,subcellular location:Nuclear, in a punctate pattern.,subunit:Homodimer, and heterodimer with SLC9A3R1. Binds PODXL and PDZK1 (By similarity). Binds ADRB2, SLC9A3, P2RY1, P2YR2, SRY, RDX and LPAR2.,tissue specificity:Widely expressed.,
- 细胞定位：
- Endomembrane system ; Peripheral membrane protein . Nucleus . Apical cell membrane . Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes (By similarity). Nuclear, in a punctate pattern. .
- Western blot analysis of JK K562 using NHERF-2 antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
