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CD47 Polyclonal Antibody

货号：YT5509
应用：WB;IHC;IF;ELISA
种属：Human;Rat;Mouse;

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靶点：
CD47

简介：
>>ECM-receptor interaction

基因名称：
CD47

蛋白名称：
Leukocyte surface antigen CD47

Human Gene Id：
961

Human Swiss Prot No：
Q08722

Mouse Swiss Prot No：
Q61735

免疫原：
The antiserum was produced against synthesized peptide derived from the Internal region of human CD47. AA range:61-110

特异性：
CD47 Polyclonal Antibody detects endogenous levels of CD47 protein.

组成：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源：
Polyclonal, Rabbit,IgG

稀释：
WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

纯化工艺：
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

浓度：
1 mg/ml

储存：
-15°C to -25°C/1 year(Do not lower than -25°C)

其他名称：
CD47;MER6;Leukocyte surface antigen CD47;Antigenic surface determinant protein OA3;Integrin-associated protein;IAP;Protein MER6;CD47

实测条带：
35kD

背景：
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010],

功能：
function:Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:Interacts with THBS1 and fibrinogen (By similarity). Monomer. Inter

细胞定位：
Cell membrane ; Multi-pass membrane protein .

组织表达：
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Suppression of the hyaluronic acid pathway induces M1 macrophages polarization via STAT1 in glioblastoma. Cell Death Discovery2022 Apr;8(1):1-13. Human U937 monocytes,U251 cell,LN229 cell
货号：YT5509