Apaf-1 Polyclonal Antibody

    • 货号:YT5378
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • APAF1
      • 简介:
      • >>Platinum drug resistance;>>p53 signaling pathway;>>Apoptosis;>>Apoptosis - multiple species;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Legionellosis;>>Tuberculosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Influenza A;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Small cell lung cancer;>>Lipid and atherosclerosis
      • 基因名称:
      • APAF1
      • 蛋白名称:
      • Apoptotic protease-activating factor 1
      • Human Gene Id:
      • 317
      • Human Swiss Prot No:
      • O14727
      • Mouse Swiss Prot No:
      • O88879
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550
      • 特异性:
      • Apaf-1 Polyclonal Antibody detects endogenous levels of Apaf-1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • APAF1;KIAA0413;Apoptotic protease-activating factor 1;APAF-1
      • 实测条带:
      • 135kD
      • 背景:
      • This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
      • 功能:
      • domain:The CARD domain mediates interaction with APIP.,function:Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.,induction:By E2F and p53 in apoptotic neurons.,similarity:Contains 1 CARD domain.,similarity:Contains 1 NB-ARC domain.,similarity:Contains 13 WD repeats.,subunit:Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP.,tissue specificity:Ubiquitous. Highest levels of expression in adult spleen and per
      • 细胞定位:
      • Cytoplasm .
      • 组织表达:
      • Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

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      Cathepsin B mediates the lysosomal-mitochondrial apoptosis pathway in arsenic-induced microglial cell injury. Baofei Sun WB Mouse 1:1000 BV2 cell
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      • 产品图片
      • Western Blot analysis of K562 cells using Apaf-1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
      • Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
      • Western blot analysis of lysate from K562 cells, using APAF1 Antibody.