• 靶点：
• Btk

• 简介：
• >>NF-kappa B signaling pathway;>>Osteoclast differentiation;>>Platelet activation;>>B cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Epstein-Barr virus infection;>>Primary immunodeficiency

• 基因名称：
• BTK

• 蛋白名称：
• Tyrosine-protein kinase BTK

• Human Gene Id：
• 695

• Human Swiss Prot No：
• Q06187

• Mouse Gene Id：
• 12229

• Mouse Swiss Prot No：
• P35991

• 免疫原：
• Synthesized peptide derived from Btk . at AA range: 490-570

• 特异性：
• Btk Polyclonal Antibody detects endogenous levels of Btk protein only when non-phosphorylation at Y550.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• BTK;AGMX1;ATK;BPK;Tyrosine-protein kinase BTK;Agammaglobulinaemia tyrosine kinase;ATK;B-cell progenitor kinase;BPK;Bruton tyrosine kinase

• 实测条带：
• 76kD

• 背景：
• The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013],

• 功能：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,disease:Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also called X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.,

• 细胞定位：
• Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.

• 组织表达：
• Predominantly expressed in B-lymphocytes.

Non-classical MHC I-E negatively regulates macrophage activation and Th17 cell development in NOD mice. Scientific Reports Sci Rep-Uk. 2015 Aug;5(1):1-9 WB Mouse BMMs
货号：YT5346

A Novel BCL-2 Inhibitor APG-2575 Exerts Synthetic Lethality With BTK or MDM2-p53 Inhibitor in Diffuse Large B-Cell Lymphoma. ONCOLOGY RESEARCH Oncol Res. 2020; 28(4): 331–344 WB Mouse OCI-LY8 xenograft tumors ,normal tissue SU-DHL 2 cell,SU-DHL 4 cell,OCI-LY 3 cell,OCI-LY 19 cell,OCI-LY 8 cell,OCI-LY 1 cell
货号：YT5346

Tatarinan N inhibits osteoclast differentiation through attenuating NF-κB, MAPKs and Ca2+-dependent signaling. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2018 Dec;65:199 WB Mouse bone marrow mononuclear cells (BMMs)
货号：YT5346

Tatarinan T, an α‐asarone‐derived lignin, attenuates osteoclastogenesis induced by RANKL via the inhibition of NFATc1/c‐Fos expression. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2019 Dec;43(12):1471-1482 WB Mouse Bone marrow monocytes (BMMs)
货号：YT5346

Yang, Chunhui, et al. "Non-classical MHC IE negatively regulates macrophage activation and Th17 cell development in NOD mice." Scientific reports 5 (2015): 12941.
货号：YT5346

A Novel BCL-2 Inhibitor APG-2575 Exerts Synthetic Lethality With BTK or MDM2-p53 Inhibitor in Diffuse Large B-Cell Lymphoma. ONCOLOGY RESEARCH Oncol Res. 2020; 28(4): 331–344 WB Mouse OCI-LY8 xenograft tumors ,normal tissue SU-DHL 2 cell,SU-DHL 4 cell,OCI-LY 3 cell,OCI-LY 19 cell,OCI-LY 8 cell,OCI-LY 1 cell
货号：YT5346

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