Pax-5 Polyclonal Antibody
- 货号：YT5312
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Transcriptional misregulation in cancer
- 蛋白名称：
- Paired box protein Pax-5
- 免疫原：
- The antiserum was produced against synthesized peptide derived from the Internal region of human PAX5. AA range:171-220
- 特异性：
- Pax-5 Polyclonal Antibody detects endogenous levels of Pax-5 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PAX5;Paired box protein Pax-5;B-cell-specific transcription factor;BSAP
- 背景：
- This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer
- 功能：
- developmental stage:Expressed at early B-cell differentiation, in the developing CNS and in adult testis.,disease:A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.,function:May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.,PTM:O-glycosylated.,similarity:Contains 1 paired domain.,subunit:Interacts with DAXX (By similarity). Binds DNA as a monomer. Binds TLE4.,
- 组织表达：
- Marginal zone lymphoma,
- Western Blot analysis of 293 cells using Pax-5 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:100
- Immunohistochemical analysis of paraffin-embedded mouse-spleen, antibody was diluted at 1:100
- Western blot analysis of lysate from 293 cells, using PAX5 Antibody.
