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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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VGF Polyclonal Antibody
- 货号:YT5296
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Neuroactive ligand-receptor interaction
- 蛋白名称:
- Neurosecretory protein VGF
- 免疫原:
- The antiserum was produced against synthesized peptide derived from the Internal region of human VGF. AA range:451-500
- 特异性:
- VGF Polyclonal Antibody detects endogenous levels of VGF protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- VGF;Neurosecretory protein VGF
- 背景:
- This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008],
- 功能:
- function:May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.,subcellular location:Stored in secretory vesicles and then secreted.,tissue specificity:Expressed in brain.,
- 细胞定位:
- [Neurosecretory protein VGF]: Secreted . Cytoplasmic vesicle, secretory vesicle . Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
- 组织表达:
- Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
- Western Blot analysis of 293 cells using VGF Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100
- Western blot analysis of lysate from 293 cells, using VGF Antibody.