SNAP 23 Polyclonal Antibody
- 货号：YT5231
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>SNARE interactions in vesicular transport;>>Platelet activation
- 蛋白名称：
- Synaptosomal-associated protein 23
- 免疫原：
- The antiserum was produced against synthesized peptide derived from the C-terminal region of human SNAP23. AA range:151-200
- 特异性：
- SNAP 23 Polyclonal Antibody detects endogenous levels of SNAP 23 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SNAP23;Synaptosomal-associated protein 23;SNAP-23;Vesicle-membrane fusion protein SNAP-23
- 背景：
- Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the
- 功能：
- function:Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.,similarity:Belongs to the SNAP-25 family.,similarity:Contains 2 t-SNARE coiled-coil homology domains.,subcellular location:Mainly localized to the plasma membrane.,subunit:Binds simultaneously to SNAP25BP and SYN4. Found in a complex with VAMP8 and STX4 in pancreas (By similarity). Binds tightly to multiple syntaxins and synaptobrevins/VAMPs. Found in a complex with VAMP8 and STX1A.,tissue specificity:Ubiquitous. Highest levels where found in placenta.,
- 细胞定位：
- Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
- 组织表达：
- Ubiquitous. Highest levels where found in placenta.
- Western Blot analysis of HepG2 cells using SNAP 23 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of lysate from HepG2 cells, using SNAP23 Antibody.
