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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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NTT4 Polyclonal Antibody
- 货号:YT5097
- 应用:WB;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Sodium-dependent neutral amino acid transporter SLC6A17
- 免疫原:
- Synthesized peptide derived from NTT4 . at AA range: 240-320
- 特异性:
- NTT4 Polyclonal Antibody detects endogenous levels of NTT4 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- SLC6A17;NTT4;Sodium-dependent neutral amino acid transporter SLC6A17;Sodium-dependent neurotransmitter transporter NTT4;Solute carrier family 6 member 17
- 背景:
- The SLC6 family of proteins, which includes SLC6A17, acts as specific transporters for neurotransmitters, amino acids, and osmolytes like betaine, taurine, and creatine. SLC6 proteins are sodium cotransporters that derive the energy for solute transport from the electrochemical gradient for sodium ions (Hoglund et al., 2005 [PubMed 16125675]).[supplied by OMIM, Mar 2008],
- 功能:
- function:Not yet known, orphan transporter.,similarity:Belongs to the sodium:neurotransmitter symporter (SNF) family.,
- 细胞定位:
- Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse . Localizes at synaptic junctions - at both pre- and post-synaptic sites - particularly in excitatory glutamatergic terminals. .
- Western Blot analysis of extracts from rat stomach, using NTT4 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000