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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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M6A Polyclonal Antibody
- 货号:YT5044
- 应用:WB;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Neuronal membrane glycoprotein M6-a
- 免疫原:
- Synthesized peptide derived from M6A . at AA range: 140-220
- 特异性:
- M6A Polyclonal Antibody detects endogenous levels of M6A protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- GPM6A;M6A;Neuronal membrane glycoprotein M6-a;M6a
- 背景:
- similarity:Belongs to the myelin proteolipid protein family.,
- 功能:
- similarity:Belongs to the myelin proteolipid protein family.,
- 细胞定位:
- Cell membrane ; Multi-pass membrane protein . Cell projection, axon . Cell projection, growth cone . Cell projection, dendritic spine . Cell projection, filopodium . Cell projection, neuron projection . Localizes to cholesterol-rich lipid rafts of the plasma membrane of hippocampal neurons. Localized to plasma membrane of cell bodies and neurites of hippocampal neurons. Localized in membrane protrusions (filopodia and spines) of primary hippocampal neurons (By similarity). Localized to the growth cone edge membrane of elongating axons (By similarity). .
- 组织表达:
- Brain,Brain cortex,Fetal brain,Spinal cord,
- Western Blot analysis of extracts from rat stomach, using M6A Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000