• 靶点：
• WTAP

• 基因名称：
• WTAP

• 蛋白名称：
• Pre-mRNA-splicing regulator WTAP

• Human Gene Id：
• 9589

• Human Swiss Prot No：
• Q15007

• Mouse Gene Id：
• 60532

• Mouse Swiss Prot No：
• Q9ER69

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human WTAP. AA range:321-370

• 特异性：
• WTAP Polyclonal Antibody detects endogenous levels of WTAP protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• WTAP;KIAA0105;Pre-mRNA-splicing regulator WTAP;Female-lethal(2)D homolog;hFL(2)D;WT1-associated protein;Wilms tumor 1-associating protein

• 实测条带：
• 55kD

• 背景：
• The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012],

• 功能：
• function:Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.,induction:In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the fl(2)d family.,subunit:Interacts with WT1.,tissue specificity:Ubiquitously expressed.,

• 细胞定位：
• Nucleus speckle . Nucleus, nucleoplasm . Cytoplasm . Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus. .

• 组织表达：
• Ubiquitously expressed.

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