WDHD1 Polyclonal Antibody
- 货号：YT4902
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 蛋白名称：
- WD repeat and HMG-box DNA-binding protein 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human WDHD1. AA range:721-770
- 特异性：
- WDHD1 Polyclonal Antibody detects endogenous levels of WDHD1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- WDHD1;AND1;WD repeat and HMG-box DNA-binding protein 1;Acidic nucleoplasmic DNA-binding protein 1;And-1
- 背景：
- The protein encoded by this gene contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
- 功能：
- caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Binds DNA with high affinity. May also be involved in protein-protein interactions.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 HMG box DNA-binding domain.,similarity:Contains 7 WD repeats.,
- 细胞定位：
- Nucleus, nucleoplasm .
- 组织表达：
- Embryonal rhabdomyosarcoma,Epithelium,Fetal brain,Liver,Ski
- Western Blot analysis of various cells using WDHD1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of lysates from Jurkat and HepG2 cells, using WDHD1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
