Tetraspanin-8 Polyclonal Antibody
- 货号：YT4609
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human TSPAN8. AA range:96-145
- 特异性：
- Tetraspanin-8 Polyclonal Antibody detects endogenous levels of Tetraspanin-8 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- TSPAN8;TM4SF3;Tetraspanin-8;Tspan-8;Transmembrane 4 superfamily member 3;Tumor-associated antigen CO-029
- 背景：
- The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008],
- 功能：
- similarity:Belongs to the tetraspanin (TM4SF) family.,tissue specificity:Gastric, colon, rectal, and pancreatic carcinomas.,
- 细胞定位：
- Membrane; Multi-pass membrane protein.
- 组织表达：
- Gastric, colon, rectal, and pancreatic carcinomas.
- Western Blot analysis of various cells using Tetraspanin-8 Polyclonal Antibody diluted at 1:1000
- Immunofluorescence analysis of HeLa cells, using TSPAN8 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from 293 and COLO cells, using TSPAN8 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
