• 靶点：
• TCF-4/12

• 基因名称：
• TCF4/TCF12

• 蛋白名称：
• Transcription factor 4/12

• Human Gene Id：
• 6925/6938

• Human Swiss Prot No：
• P15884/Q99081

• Mouse Gene Id：
• 21413/21406

• Rat Gene Id：
• 84382/25720

• Rat Swiss Prot No：
• Q62655/P51514

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human TCF4/12. AA range:581-630

• 特异性：
• TCF-4/12 Polyclonal Antibody detects endogenous levels of TCF-4/12 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• TCF4;BHLHB19;ITF2;SEF2;Transcription factor 4;TCF-4;Class B basic helix-loop-helix protein 19;bHLHb19;Immunoglobulin transcription factor 2;ITF-2;SL3-3 enhancer factor 2;SEF-2;TCF12;BHLHB20;HEB;HTF4;Transcription factor 12;

• 实测条带：
• 60kD

• 背景：
• This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016],

• 功能：
• disease:Defects in TCF4 are a cause of Pitt-Hopkins syndrome (PTHS) [MIM:610954].,disease:Haploinsufficiency of TCF4 is a cause of Pitt-Hopkins syndrome (PTHS) [MIM:610954]. PTHS is a rare syndromic encephalopathy characterized by severe psychomotor delay, epilepsy, daily bouts of diurnal hyperventilation starting in infancy, mild postnatal growth retardation, postnatal microcephaly, and distinctive facial features. Since most hitherto reported cases have been sporadic, with males and females equally affected, PTHS is regarded as an autosomal dominant condition.,function:Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.,sequence caution:Incomplete and probable erro

• 细胞定位：
• Nucleus .

• 组织表达：
• Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.

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MicroRNA‑190b expression predicts a good prognosis and attenuates the malignant progression of pancreatic cancer by targeting MEF2C and TCF4. ONCOLOGY REPORTS Oncol Rep. 2022 Jan;47(1):1-13 WB，IHC Human 1:1000,1:300 PDAC tissues,matched non-tumor adjacent tissue Parental AsPC-1 cell
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TCF21 functions as a tumor suppressor in colorectal cancer through inactivation of PI3K/AKT signaling. OncoTargets and Therapy Oncotargets Ther. 2017; 10: 1603–1611 WB Human colorectal epithelial tissues
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