TCF-3 Polyclonal Antibody
- 货号:YT4579
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Signaling pathways regulating pluripotency of stem cells;>>Human T-cell leukemia virus 1 infection;>>Transcriptional misregulation in cancer
- 蛋白名称:
- Transcription factor E2-alpha
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TCF3. AA range:571-620
- 特异性:
- TCF-3 Polyclonal Antibody detects endogenous levels of TCF-3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TCF3;BHLHB21;E2A;ITF1;Transcription factor E2-alpha;Class B basic helix-loop-helix protein 21;bHLHb21;Immunoglobulin enhancer-binding factor E12/E47;Immunoglobulin transcription factor 1;Kappa-E2-binding factor;Transcription facto
- 背景:
- This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have bee
- 功能:
- disease:Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.,function:Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.,PTM:Phosphorylated following NGF stimulation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with ASH1 and TWIST2. Isoform E12 interacts with GRIPE and FIGLA (By similarity). Interacts with PTF1A and TGFB1I1.
- 组织表达:
- Lymphoma,Muscle,PCR rescued clones,
![](/uploadfiles/PIC/yt4579-wb.jpg)
- Western Blot analysis of various cells using TCF-3 Polyclonal Antibody
![](/uploadfiles/PIC/yt4579_pic506318522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TCF3 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt4579_pic506418522.jpg)
- Western blot analysis of lysates from 293 cells, using TCF3 Antibody. The lane on the right is blocked with the synthesized peptide.