• 靶点：
• TCF-1

• 简介：
• >>Wnt signaling pathway;>>Hippo signaling pathway;>>Adherens junction;>>Signaling pathways regulating pluripotency of stem cells;>>Melanogenesis;>>Cushing syndrome;>>Alcoholic liver disease;>>Salmonella infection;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Pathways in cancer;>>Colorectal cancer;>>Endometrial cancer;>>Prostate cancer;>>Thyroid cancer;>>Basal cell carcinoma;>>Acute myeloid leukemia;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer;>>Arrhythmogenic right ventricular cardiomyopathy

• 基因名称：
• TCF7

• 蛋白名称：
• Transcription factor 7

• Human Gene Id：
• 6932

• Human Swiss Prot No：
• P36402

• Mouse Gene Id：
• 21414

• Mouse Swiss Prot No：
• Q00417

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human TCF7. AA range:10-59

• 特异性：
• TCF-1 Polyclonal Antibody detects endogenous levels of TCF-1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• TCF7;TCF1;Transcription factor 7;TCF-7;T-cell-specific transcription factor 1;T-cell factor 1;TCF-1

• 实测条带：
• 42kD

• 背景：
• alternative products:2 series of isoforms, L and S, are produced by use of alternative promoter usage. Additional isoforms seem to exist,function:Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.,induction:By TCF7L2 and CTNNB1.,sequence caution:Wrong choice of frame.,similarity:Belongs to the TCF/LEF family.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4.,tissue specificity:Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.,

• 功能：
• alternative products:2 series of isoforms, L and S, are produced by use of alternative promoter usage. Additional isoforms seem to exist,function:Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.,induction:By TCF7L2 and CTNNB1.,sequence caution:Wrong choice of frame.,similarity:Belongs to the TCF/LEF family.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1,

• 细胞定位：
• Nucleus.

• 组织表达：
• Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.

Nigericin Exerts Anticancer Effects on Human Colorectal Cancer Cells by Inhibiting Wnt/β-catenin Signaling Pathway. MOLECULAR CANCER THERAPEUTICS Mol Cancer Ther. 2018 May;17(5):952-965 WB Human SW620 cell，KM12 cell
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