• 靶点：
• Tau

• 简介：
• >>MAPK signaling pathway;>>Alzheimer disease;>>Parkinson disease;>>Pathways of neurodegeneration - multiple diseases

• 基因名称：
• MAPT

• 蛋白名称：
• Microtubule-associated protein tau

• Human Gene Id：
• 4137

• Human Swiss Prot No：
• P10636

• Mouse Gene Id：
• 17762

• Mouse Swiss Prot No：
• P10637

• Rat Swiss Prot No：
• P19332

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human Tau. AA range:496-545

• 特异性：
• Tau Polyclonal Antibody detects endogenous levels of Tau protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• MAPT;MAPTL;MTBT1;TAU;Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau

• 实测条带：
• 50-85kD

• 背景：
• This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008],

• 功能：
• alternative products:Additional isoforms seem to exist. Isoforms differ from each other by the presence or absence of up to 5 of the 15 exons. One of these optional exons contains the additional tau/MAP repeat,developmental stage:Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.,disease:Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.,disease:Defects in MAPT are a cause of frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP17) [MIM:600274, 172700]; also called frontotemporal dementia (FTD) or historically termed Pick complex. This form

• 细胞定位：
• Cytoplasm, cytosol . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell projection, axon . Cell projection, dendrite . Secreted . Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components (PubMed:10747907). Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059). .

• 组织表达：
• Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

Integrating network pharmacology, UPLC-Q–TOF–MS and molecular docking to investigate the effect and mechanism of Chuanxiong Renshen decoction against Alzheimer's disease Chinese Medicine Shen, Zhuo Jun, Fu, Yun Bo, Hou, Jin Ling, Lin, Lu Ning, Wang, Xiao Yan, Li, Chang Yu, Yang, Yuan Xiao WB Mouse brain
货号：YT4546

Icariin Ameliorates Alzheimer’s Disease Pathology by Alleviating Myelin Injury in 3 × Tg-AD Mice Neurochem Res. 2022 Jan;:1-11. WB Mouse hippocampus
货号：YT4546

Leptin signaling plays a critical role in the geniposide-induced decrease of tau phosphorylation. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2015 Dec 01 WB Mouse Hemibrain
货号：YT4546

Epigallocatechin-3-gallate Alleviates Cognitive Deficits in APP/PS1 Mice. Current Medical Science 2020 Mar 13 WB Mouse 1:1000 Hippocampi ,cortexes
货号：YT4546

Activation of mTOR signaling leads to orthopedic surgery-induced cognitive decline in mice through β-amyloid accumulation and tau phosphorylation. Molecular Medicine Reports 2016 Sep 01 WB Mouse 1:500 hippocampal tissues
货号：YT4546

Fu, Yunbo, et al. "Acori tatarinowii rhizoma extract ameliorates Alzheimer's pathological syndromes by repairing myelin injury and lowering Tau phosphorylation in mice." Die Pharmazie-An International Journal of Pharmaceutical Sciences 75.8 (2020): 395-400.
货号：YT4546

Essential oil of Acorus tatarinowii Schott inhibits neuroinflammation by suppressing NLRP3 inflammasome activation in 3 × Tg-AD transgenic mice PHYTOMEDICINE Changyu Li WB Mouse hippocampus tissues
货号：YT4546

Icariin, a natural flavonoid glucoside, inhibits neuroinflammation in mice with triple-transgenic Alzheimer’s disease by regulating the Akt/GSK-3β signaling pathway Journal of Functional Foods Sichen Wu WB Mouse cerebral cortex tissue
货号：YT4546

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs