T2R10 Polyclonal Antibody
- 货号：YT4504
- 应用：WB;ELISA;IHC
- 种属：Human
- 蛋白名称：
- Taste receptor type 2 member 10
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human TAS2R10. AA range:122-171
- 特异性：
- T2R10 Polyclonal Antibody detects endogenous levels of T2R10 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- TAS2R10;Taste receptor type 2 member 10;T2R10;Taste receptor family B member 2;TRB2
- 背景：
- This gene product belongs to the family of candidate taste receptors that are members of the G-protein-coupled receptor superfamily. These proteins are specifically expressed in the taste receptor cells of the tongue and palate epithelia. They are organized in the genome in clusters and are genetically linked to loci that influence bitter perception in mice and humans. In functional expression studies, they respond to bitter tastants. This gene maps to the taste receptor gene cluster on chromosome 12p13. [provided by RefSeq, Jul 2008],
- 功能：
- function:Gustducin-coupled strychnine receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5.,miscellaneous:Several bitter taste receptors are expressed in a single taste receptor cell.,similarity:Belongs to the G-protein coupled receptor T2R family.,tissue specificity:Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.,
- 细胞定位：
- Membrane; Multi-pass membrane protein.
- 组织表达：
- Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.
- Immunofluorescence analysis of MCF7 cells, using TAS2R10 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from LOVO cells, using TAS2R10 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using TAS2R10 antibody.
- Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
