SURF-1 Polyclonal Antibody
- 货号:YT4471
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Surfeit locus protein 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human SURF1. AA range:171-220
- 特异性:
- SURF-1 Polyclonal Antibody detects endogenous levels of SURF-1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- SURF1;SURF-1;Surfeit locus protein 1
- 背景:
- This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency. [provided by RefSeq, Jul 2008],
- 功能:
- disease:Defects in SURF1 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions that is commonly associated with systemic cytochrome c oxidase (COX) deficiency.,function:Probably involved in the biogenesis of the COX complex.,similarity:Belongs to the SURF1 family.,
- 细胞定位:
- Mitochondrion inner membrane ; Multi-pass membrane protein .
- 组织表达:
- Colon,Kidney,Skin,Stomach,
- Western Blot analysis of various cells using SURF-1 Polyclonal Antibody
- Western Blot analysis of Jurkat cells using SURF-1 Polyclonal Antibody
- Western blot analysis of lysates from Jurkat cells, using SURF1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).