SRp75 Polyclonal Antibody
- 货号：YT4421
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Spliceosome;>>Herpes simplex virus 1 infection
- 蛋白名称：
- Serine/arginine-rich splicing factor 4
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human SFRS4. AA range:111-160
- 特异性：
- SRp75 Polyclonal Antibody detects endogenous levels of SRp75 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SRSF4;SFRS4;SRP75;Serine/arginine-rich splicing factor 4;Pre-mRNA-splicing factor SRP75;SRP001LB;Splicing factor; arginine/serine-rich 4
- 背景：
- This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. [provided by RefSeq, Feb 2009],
- 功能：
- function:A probable role in alternative splice site selection during pre-mRNA splicing.,PTM:Extensively phosphorylated on serine residues in the RS domain.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 2 RRM (RNA recognition motif) domains.,subunit:Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Interacts with PNN.,
- 组织表达：
- Aorta endothelial cell,Cerebrum,Epithelium,Lymph,
- Western Blot analysis of various cells using SRp75 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Human thyroid gland. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from LOVO cells, using SFRS4 Antibody. The lane on the right is blocked with the synthesized peptide.
