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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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SPZ1 Polyclonal Antibody
- 货号:YT4390
- 应用:IHC;IF;WB;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- Spermatogenic leucine zipper protein 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human SPZ1. AA range:111-160
- 特异性:
- SPZ1 Polyclonal Antibody detects endogenous levels of SPZ1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- SPZ1;TSP1;Spermatogenic leucine zipper protein 1;Testis-specific protein 1;Testis-specific protein NYD-TSP1
- 背景:
- This gene encodes a bHLH-zip transcription factor which functions in the mitogen-activate protein kinase (MAPK) signaling pathway. Because of its role in the upregulation of cell proliferation and tumorigenesis, this gene may serve as a target for Ras-induced tumor treatments. [provided by RefSeq, Oct 2011],
- 功能:
- function:Transcription factor that binds to the DNA sequence 5'-CANNTG-3'(E box) and the G-box motif. May play an important role in the regulation of cell proliferation and differentiation during spermatogenesis.,PTM:Phosphorylated by ERK1 and ERK2.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Interacts with PPP1CC isoform gamma-2.,tissue specificity:Specifically and strongly expressed in the testis. Expressed in several tumor cell lines.,
- 细胞定位:
- Cytoplasm . Nucleus .
- 组织表达:
- Specifically and strongly expressed in the testis. Expressed in several tumor cell lines.
![](/uploadfiles/PIC/yt4390_pic573818522.jpg)
- Immunofluorescence analysis of A549 cells, using SPZ1 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt4390_pic573918522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human testis tissue, using SPZ1 Antibody. The picture on the right is blocked with the synthesized peptide.