Septin 14 Polyclonal Antibody
- 货号:YT4246
- 应用:WB;IHC
- 种属:Human;Mouse
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human SEPT14. AA range:341-390
- 特异性:
- Septin 14 Polyclonal Antibody detects endogenous levels of Septin 14 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- SEPT14 is a member of the highly conserved septin family of GTP-binding cytoskeletal proteins implicated in membrane transport, apoptosis, cell polarity, cell cycle regulation, cytokinesis, and other cellular functions (Peterson et al., 2007 [PubMed 17922164]).[supplied by OMIM, Jul 2008],
- 功能:
- function:Involved in cytokinesis .,similarity:Belongs to the septin family.,
- 细胞定位:
- Cytoplasm . Cytoplasm, cytoskeleton . Cell projection, axon . Cell projection, dendrite . Perikaryon . Cytoplasm, perinuclear region . Cytoplasmic vesicle, secretory vesicle, acrosome . Colocalizes with actin stress fibers (PubMed:17922164). Expressed in the perinuclear rim and manchette structure in early elongating spermatids during spermiogenesis (By similarity). .
- 组织表达:
- Testis-specific (at protein level).
- Western Blot analysis of 3T3 cells using Septin 14 Polyclonal Antibody
- Western blot analysis of SEPT14 Antibody. The lane on the right is blocked with the SEPT14 peptide.
- Western blot analysis of the lysates from Jurkat cells using SEPT14 antibody.
- Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).