Septin 1 Polyclonal Antibody
- 货号:YT4245
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Bacterial invasion of epithelial cells
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human SEPT1. AA range:181-230
- 特异性:
- Septin 1 Polyclonal Antibody detects endogenous levels of Septin 1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- SEPT1;DIFF6;PNUTL3;Septin-1;LARP;Peanut-like protein 3;Serologically defined breast cancer antigen NY-BR-24
- 背景:
- septin 1(SEPT1) Homo sapiens This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012],
- 功能:
- function:Involved in cytokinesis .,similarity:Belongs to the septin family.,subunit:May assemble into a multicomponent structure.,
- 细胞定位:
- Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody. Remains at the centrosomes and the nearby microtubules throughout mitosis. Localizes to the midbody during cytokinesis.
- 组织表达:
- Expressed at high levels in lymphoid and hematopoietic tissues.
- Western Blot analysis of various cells using Septin 1 Polyclonal Antibody
- Western blot analysis of lysates from Jurkat cells, using SEPT1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200