SAPAP1 Polyclonal Antibody
- 货号:YT4212
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Glutamatergic synapse
- 蛋白名称:
- Disks large-associated protein 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human DLGP1. AA range:791-840
- 特异性:
- SAPAP1 Polyclonal Antibody detects endogenous levels of SAPAP1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DLGAP1;DAP1;GKAP;Disks large-associated protein 1;DAP-1;Guanylate kinase-associated protein;hGKAP;PSD-95/SAP90-binding protein 1;SAP90/PSD-95-associated protein 1;SAPAP1
- 背景:
- function:Part of the postsynaptic scaffold in neuronal cells.,similarity:Belongs to the SAPAP family.,subcellular location:Found in postsynaptic density of neuronal cells.,subunit:Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.,tissue specificity:Expressed in brain.,
- 功能:
- function:Part of the postsynaptic scaffold in neuronal cells.,similarity:Belongs to the SAPAP family.,subcellular location:Found in postsynaptic density of neuronal cells.,subunit:Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.,tissue specificity:Expressed in brain.,
- 细胞定位:
- Cell membrane ; Peripheral membrane protein . Cell junction, synapse, postsynaptic density . Cell junction, synapse .
- 组织表达:
- Expressed in brain.
- Western Blot analysis of various cells using SAPAP1 Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using DLGP1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HepG2 cells, using DLGP1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).