S-100A16 Polyclonal Antibody
- 货号:YT4199
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human S100A16. AA range:19-68
- 特异性:
- S-100A16 Polyclonal Antibody detects endogenous levels of S-100A16 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- S100A16;S100F;AAG13;Protein S100-A16;Aging-associated gene 13 protein;Protein S100-F;S100 calcium-binding protein A16
- 背景:
- similarity:Belongs to the S-100 family.,similarity:Contains 2 EF-hand domains.,
- 功能:
- similarity:Belongs to the S-100 family.,similarity:Contains 2 EF-hand domains.,
- 细胞定位:
- Nucleus, nucleolus . Cytoplasm . Primarily nucleolar. A high intracellular calcium level induces nucleolar exit and nucleocytoplasmic transport, whereas a low intracellular calcium level leads to nuclear translocation and accumulation within specific region of nucleoli (PubMed:17030513).
- 组织表达:
- Ubiquitous (PubMed:14684152). Highly expressed in esophagus, adipose tissues and colon. Expressed at lower level in lung, brain, pancreas and skeletal muscle. Expression is up-regulated in tumors of bladder, lung, thyroid gland, pancreas and ovary (PubMed:14684152). Expressed in astrocytes (PubMed:17030513).
![](/uploadfiles/PIC/yt4199-ihc-176.jpg)
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
![](/uploadfiles/PIC/yt4199-ihc-977.jpg)
- Immunohistochemical analysis of paraffin-embedded Human cervix cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
![](/uploadfiles/PIC/yt4199_pic738118522.jpg)
- Western blot analysis of lysates from NIH/3T3 and RAW264.7 cells, using S100A16 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt4199_pic1056818522.jpg)
- Western blot analysis of the lysates from RAW264.7cells using S100A16 antibody.