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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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Rrn3 Polyclonal Antibody
- 货号:YT4176
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- RNA polymerase I-specific transcription initiation factor RRN3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TIF-IA. AA range:602-651
- 特异性:
- Rrn3 Polyclonal Antibody detects endogenous levels of Rrn3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RRN3;TIFIA;RNA polymerase I-specific transcription initiation factor RRN3;Transcription initiation factor IA;TIF-IA
- 背景:
- function:Required for efficient transcription initiation by RNA polymerase I.,similarity:Belongs to the RRN3 family.,
- 功能:
- function:Required for efficient transcription initiation by RNA polymerase I.,similarity:Belongs to the RRN3 family.,
- 细胞定位:
- Nucleus, nucleolus .
- 组织表达:
- Hippocampus,Kidney,Pooled,Spleen,
![](/uploadfiles/PIC/yt4176_pic425018522.jpg)
- Western blot analysis of lysates from HeLa cells, treated with calyculinA 50ng/ml 30', using TIF-IA Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/6ihcbrainn20220713.jpg)
- Immunohistochemical analysis of paraffin-embedded human brain. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).