Ribosomal Protein L18 Polyclonal Antibody

    • 货号:YT4099
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Ribosomal Protein L18
      • 简介:
      • >>Ribosome;>>Coronavirus disease - COVID-19
      • 基因名称:
      • RPL18
      • 蛋白名称:
      • 60S ribosomal protein L18
      • Human Gene Id:
      • 6141
      • Human Swiss Prot No:
      • Q07020
      • Mouse Swiss Prot No:
      • P35980
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human RPL18. AA range:111-160
      • 特异性:
      • Ribosomal Protein L18 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L18 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • RPL18;60S ribosomal protein L18
      • 实测条带:
      • 23kD
      • 背景:
      • Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012],
      • 功能:
      • similarity:Belongs to the ribosomal protein L18e family.,
      • 细胞定位:
      • Cytoplasm, cytosol . Cytoplasm . Rough endoplasmic reticulum . Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity). .
      • 组织表达:
      • Brain,Colon,Epithelium,Lung,
      • 产品图片
      • Western Blot analysis of various cells using Ribosomal Protein L18 Polyclonal Antibody diluted at 1:2000
      • Western blot analysis of lysates from HeLa and COLO cells, using RPL18 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HeLa cells using RPL18 antibody.
      • Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).