- 靶点：
- Renin
- 简介：
- >>Renin-angiotensin system;>>Renin secretion;>>Diabetic cardiomyopathy
- 基因名称：
- REN
- 蛋白名称：
- Renin
- Human Gene Id：
- 5972
- Human Swiss Prot No：
- P00797
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human REN. AA range:207-256
- 特异性：
- Renin Polyclonal Antibody detects endogenous levels of Renin protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- REN;Renin;Angiotensinogenase
- 实测条带：
- 40kD
- 背景：
- Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.,disease:Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).,enzyme regulation:Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.,function:Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.,online information:Renin entry,similarity:Belongs to the peptidase A1 family.,subcellular location:Associated to
- 细胞定位：
- Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
- 组织表达：
- Colon,Fetal liver,Ovary,
Increased abundance of bacteria of the family Muribaculaceae achieved by fecal microbiome transplantation correlates with the inhibition of kidney calcium oxalate stone deposition in experimental rats. Pan TieJun IHC Rat kidney tissue
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- Antibody-FAQs
- 产品图片
- Western blot analysis of lysates from Jurkat cells, using REN Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using REN antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
