• 靶点：
• RECK

• 简介：
• >>MicroRNAs in cancer

• 基因名称：
• RECK

• 蛋白名称：
• Reversion-inducing cysteine-rich protein with Kazal motifs

• Human Gene Id：
• 8434

• Human Swiss Prot No：
• O95980

• Mouse Gene Id：
• 53614

• Mouse Swiss Prot No：
• Q9Z0J1

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human RECK. AA range:21-70

• 特异性：
• RECK Polyclonal Antibody detects endogenous levels of RECK protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• RECK;ST15;Reversion-inducing cysteine-rich protein with Kazal motifs;hRECK;Suppressor of tumorigenicity 15 protein

• 实测条带：
• 110kD

• 背景：
• The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015],

• 功能：
• function:Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.,PTM:N-glycosylated.,similarity:Contains 3 Kazal-like domains.,subunit:Interacts with MMP-9.,tissue specificity:Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.,

• 细胞定位：
• Cell membrane ; Lipid-anchor, GPI-anchor .

• 组织表达：
• Expressed in various tissues and untransformed cells (PubMed:9789069). It is undetectable in tumor-derived cell lines and oncogenically transformed cells (PubMed:9789069).

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