Rab 5C Polyclonal Antibody
- 货号:YT3936
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Ras signaling pathway;>>Endocytosis;>>Phagosome;>>Vasopressin-regulated water reabsorption;>>Salmonella infection;>>Amoebiasis;>>Tuberculosis
- 蛋白名称:
- Ras-related protein Rab-5C
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human RAB5C. AA range:122-171
- 特异性:
- Rab 5C Polyclonal Antibody detects endogenous levels of Rab 5C protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RAB5C;RABL;Ras-related protein Rab-5C;L1880;RAB5L
- 背景:
- Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).[supplied by OMIM, Nov 2010],
- 功能:
- function:Protein transport. Probably involved in vesicular traffic.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Binds EEA1.,
- 细胞定位:
- Cell membrane ; Lipid-anchor ; Cytoplasmic side . Early endosome membrane ; Lipid-anchor . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
- Western blot analysis of lysates from LOVO, HeLa, and Jurkat cells, using RAB5C Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from 293 cells using RAB5C antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).