• 靶点：
• PRP19

• 简介：
• >>Spliceosome;>>Ubiquitin mediated proteolysis

• 基因名称：
• PRPF19

• 蛋白名称：
• Pre-mRNA-processing factor 19

• Human Gene Id：
• 27339

• Human Swiss Prot No：
• Q9UMS4

• Mouse Gene Id：
• 28000

• Mouse Swiss Prot No：
• Q99KP6

• Rat Gene Id：
• 246216

• Rat Swiss Prot No：
• Q9JMJ4

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human PRPF19. AA range:171-220

• 特异性：
• PRP19 Polyclonal Antibody detects endogenous levels of PRP19 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• PRPF19;NMP200;PRP19;SNEV;Pre-mRNA-processing factor 19;Nuclear matrix protein 200;PRP19/PSO4 homolog;hPso4;Senescence evasion factor

• 实测条带：
• 50kD

• 背景：
• PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM, Sep 2008],

• 功能：
• function:Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.,induction:By gamma radiation and chemical mutagens but not by UV treatment.,similarity:Belongs to the WD repeat PRP19 family.,similarity:Contains 1 U-box domain.,similarity:Contains 7 WD repeats.,subcellular locatio

• 细胞定位：
• Nucleus . Nucleus, nucleoplasm . Cytoplasm, cytoskeleton, spindle . Cytoplasm . Lipid droplet . Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin. Recruited on damaged DNA at sites of double-strand break. .

• 组织表达：
• Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.

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