Prefoldin 1 Polyclonal Antibody
- 货号:YT3854
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Prefoldin subunit 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human PFDN1. AA range:1-50
- 特异性:
- Prefoldin 1 Polyclonal Antibody detects endogenous levels of Prefoldin 1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PFDN1;PFD1;Prefoldin subunit 1
- 背景:
- This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq, Jul 2008],
- 功能:
- function:Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.,similarity:Belongs to the prefoldin subunit beta family.,subunit:Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.,
![](/uploadfiles/PIC/yt3854_pic490418522.jpg)
- Western blot analysis of lysates from RAW264.7 cells, using PFDN1 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/113biantaoticc.jpg)
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).