PITPβ Polyclonal Antibody

    • 货号:YT3738
    • 应用:WB;ELISA;IHC
    • 种属:Human;Mouse;Rat
      • 靶点:
      • PITPβ
      • 基因名称:
      • PITPNB
      • 蛋白名称:
      • Phosphatidylinositol transfer protein beta isoform
      • Human Swiss Prot No:
      • P48739
      • Mouse Swiss Prot No:
      • P53811
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human PITPNB. AA range:20-69
      • 特异性:
      • PITPβ Polyclonal Antibody detects endogenous levels of PITPβ protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • PITPNB;Phosphatidylinositol transfer protein beta isoform;PI-TP-beta;PtdIns transfer protein beta;PtdInsTP beta
      • 实测条带:
      • 32kD
      • 背景:
      • This gene encodes a cytoplasmic protein that catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes. This transfer activity is required for COPI complex-mediated retrograde transport from the Golgi apparatus to the endoplasmic reticulum. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013],
      • 功能:
      • function:Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.,PTM:Constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity but is required for Golgi targeting.,similarity:Belongs to the PtdIns transfer protein family. Class I subfamily.,tissue specificity:Widely expressed in various tissues including brain.,
      • 细胞定位:
      • Golgi apparatus . Golgi apparatus membrane . Endoplasmic reticulum membrane .
      • 组织表达:
      • Widely expressed in various tissues including brain.
      • 产品图片
      • Western Blot analysis of various cells using PITPβ Polyclonal Antibody diluted at 1:2000
      • Western Blot analysis of HepG2 cells using PITPβ Polyclonal Antibody diluted at 1:2000
      • Western blot analysis of lysates from HepG2 cells, using PITPNB Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from K562 cells using PITPNB antibody.
      • Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).