PITPβ Polyclonal Antibody
- 货号:YT3738
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Phosphatidylinositol transfer protein beta isoform
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human PITPNB. AA range:20-69
- 特异性:
- PITPβ Polyclonal Antibody detects endogenous levels of PITPβ protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PITPNB;Phosphatidylinositol transfer protein beta isoform;PI-TP-beta;PtdIns transfer protein beta;PtdInsTP beta
- 背景:
- This gene encodes a cytoplasmic protein that catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes. This transfer activity is required for COPI complex-mediated retrograde transport from the Golgi apparatus to the endoplasmic reticulum. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013],
- 功能:
- function:Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.,PTM:Constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity but is required for Golgi targeting.,similarity:Belongs to the PtdIns transfer protein family. Class I subfamily.,tissue specificity:Widely expressed in various tissues including brain.,
- 细胞定位:
- Golgi apparatus . Golgi apparatus membrane . Endoplasmic reticulum membrane .
- 组织表达:
- Widely expressed in various tissues including brain.
- Western Blot analysis of various cells using PITPβ Polyclonal Antibody diluted at 1:2000
- Western Blot analysis of HepG2 cells using PITPβ Polyclonal Antibody diluted at 1:2000
- Western blot analysis of lysates from HepG2 cells, using PITPNB Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from K562 cells using PITPNB antibody.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).