PHAPI2 Polyclonal Antibody
- 货号：YT3695
- 应用：WB;ELISA;IHC
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Acidic leucine-rich nuclear phosphoprotein 32 family member B
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ANP32B. AA range:1-50
- 特异性：
- PHAPI2 Polyclonal Antibody detects endogenous levels of PHAPI2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ANP32B;APRIL;PHAPI2;Acidic leucine-rich nuclear phosphoprotein 32 family member B;Acidic protein rich in leucines;Putative HLA-DR-associated protein I-2;PHAPI2;Silver-stainable protein SSP29
- 背景：
- function:Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.,similarity:Belongs to the ANP32 family.,similarity:Contains 4 LRR (leucine-rich) repeats.,subcellular location:Accumulates in the nuclei at the S phase.,tissue specificity:Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.,
- 功能：
- function:Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.,similarity:Belongs to the ANP32 family.,similarity:Contains 4 LRR (leucine-rich) repeats.,subcellular location:Accumulates in the nuclei at the S phase.,tissue specificity:Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.,
- 细胞定位：
- [Isoform 1]: Nucleus . Cytoplasm . Accumulates in the nuclei at the S phase. .; [Isoform 2]: Cytoplasm. Lacks a nuclear localization signal.
- 组织表达：
- Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.
- Western Blot analysis of rat cells using PHAPI2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from rat brain cells, using ANP32B Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using ANP32B antibody.
- Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
