- 靶点:
- PERK
- 简介:
- >>Mitophagy - animal;>>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Hepatitis C;>>Measles;>>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis
- 基因名称:
- EIF2AK3
- 蛋白名称:
- Eukaryotic translation initiation factor 2-alpha kinase 3
- Human Gene Id:
- 9451
- Human Swiss Prot No:
- Q9NZJ5
- Mouse Swiss Prot No:
- Q9Z2B5
- Rat Gene Id:
- 29702
- Rat Swiss Prot No:
- Q9Z1Z1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human EIF2AK3. AA range:947-996
- 特异性:
- PERK Polyclonal Antibody detects endogenous levels of PERK protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- EIF2AK3;PEK;PERK;Eukaryotic translation initiation factor 2-alpha kinase 3;PRKR-like endoplasmic reticulum kinase;Pancreatic eIF2-alpha kinase;HsPEK
- 实测条带:
- 125kD
- 背景:
- The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.,domain:The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.,enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase act
- 细胞定位:
- Endoplasmic reticulum membrane; Single-pass type I membrane protein.
- 组织表达:
- Ubiquitous. A high level expression is seen in secretory tissues.
NLRP3 inflammasome and endoplasmic reticulum stress in the epileptogenic zone in temporal lobe epilepsy: molecular insights into their interdependence. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY Neuropath Appl Neuro. 2020 Dec;46(7):770-785 WB,IF,IHC Mouse,Human 1:500,1:200,1:500 Temporal neocortices, bilateral hippocampi
货号:YT3666
Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2017 Mar 01 WB Human 1:2000 renal cancer tissues 786-O cell
货号:YT3666
Sodium fluoride induces apoptosis through reactive oxygen species-mediated endoplasmic reticulum stress pathway in Sertoli cells. Journal of Environmental Sciences 2015 Feb 24 WB Rat Sertoli cell
货号:YT3666
Activation of UPR Signaling Pathway is Associated With the Malignant Progression and Poor Prognosis in Prostate Cancer. PROSTATE 2016 Oct 08 IHC Human 1:100 Prostate cancer tissues
货号:YT3666
Cadmium induces liver dysfunction and ferroptosis through the endoplasmic stress-ferritinophagy axis ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jiacheng Wu WB Mouse
货号:YT3666
α-Hederin induces human colorectal cancer cells apoptosis through disturbing protein homeostasis. CHEMICO-BIOLOGICAL INTERACTIONS Haibo Cheng WB Human HCT116 cell
货号:YT3666
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- Antibody-FAQs
- 产品图片
- Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
- Immunofluorescence analysis of rat-lung tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Immunofluorescence analysis of rat-spleen tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Immunofluorescence analysis of mouse-lung tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Western blot analysis of lysates from MCF-7 cells, using PEK/PERK Antibody. The lane on the right is blocked with the synthesized peptide.