PC-PLD4 Polyclonal Antibody
- 货号:YT3622
- 应用:WB;IHC
- 种属:Human;Mouse;Rat
- 简介:
- >>Glycerophospholipid metabolism;>>Ether lipid metabolism;>>Metabolic pathways
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human PLD4. AA range:457-506
- 特异性:
- PC-PLD4 Polyclonal Antibody detects endogenous levels of PC-PLD4 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PLD4;C14orf175;Phospholipase D4;PLD 4;Choline phosphatase 4;Phosphatidylcholine-hydrolyzing phospholipase D4
- 背景:
- catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,similarity:Belongs to the phospholipase D family.,similarity:Contains 2 PLD phosphodiesterase domains.,
- 功能:
- catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,similarity:Belongs to the phospholipase D family.,similarity:Contains 2 PLD phosphodiesterase domains.,
- 细胞定位:
- Endoplasmic reticulum membrane ; Single-pass type II membrane protein . Golgi apparatus, trans-Golgi network membrane ; Single-pass type II membrane protein . Nucleus . Early endosome . Cytoplasmic vesicle, phagosome . Activation of microglia induces translocation of PLD4 from the nucleus to the phagosomes. .
- Western Blot analysis of Jurkat cells using PC-PLD4 Polyclonal Antibody
- Western blot analysis of lysates from Jurkat cells, using PLD4 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from K562 cells using PLD4 antibody.
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).