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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PBF Polyclonal Antibody
- 货号:YT3609
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Pituitary tumor-transforming gene 1 protein-interacting protein
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human PTTG. AA range:116-165
- 特异性:
- PBF Polyclonal Antibody detects endogenous levels of PBF protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PTTG1IP;C21orf1;C21orf3;Pituitary tumor-transforming gene 1 protein-interacting protein;Pituitary tumor-transforming gene protein-binding factor;PBF;PTTG-binding factor
- 背景:
- This gene encodes a single-pass type I integral membrane protein, which binds to pituitary tumor-transforming 1 protein (PTTG1), and facilitates translocation of PTTG1 into the nucleus. Coexpression of this protein and PTTG1 induces transcriptional activation of basic fibroblast growth factor. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013],
- 功能:
- function:May facilitate PTTG1 nuclear translocation.,induction:By transcription factor RUNX2.,subcellular location:According to PubMed:10781616 it is found in the cytoplasm and the nucleus.,subunit:Interacts with PTTG1.,tissue specificity:Ubiquitous.,
- 细胞定位:
- Membrane ; Single-pass type I membrane protein . Cytoplasm . Nucleus . According to PubMed:10781616, it is found in the cytoplasm and the nucleus.
- Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PTTG Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from RAW264.7 cells, using PTTG Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using PTTG antibody.