p53RFP Polyclonal Antibody
- 货号：YT3544
- 应用：WB;ELISA
- 种属：Human;Mouse
- 蛋白名称：
- E3 ubiquitin-protein ligase RNF144B
- 免疫原：
- Synthesized peptide derived from the Internal region of human p53RFP.
- 特异性：
- p53RFP Polyclonal Antibody detects endogenous levels of p53RFP protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- RNF144B;IBRDC2;P53RFP;E3 ubiquitin-protein ligase RNF144B;IBR domain-containing protein 2;RING finger protein 144B;p53-inducible RING finger protein
- 背景：
- caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.,pathway:Protein modification; protein ubiquitination.,PTM:Auto-ubiquitinated.,similarity:Belongs to the RBR family. RNF144 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with UBE2L3, UBE2L6 and LCMT2.,tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymus.,
- 功能：
- caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.,pathway:Protein modification; protein ubiquitination.,PTM:Auto-ubiquitinated.,similarity:Belongs to the RBR family. RNF144 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with UBE2L3, UBE2L6 and LCMT2.,tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymu
- 细胞定位：
- Mitochondrion membrane ; Single-pass membrane protein . Cytoplasm . Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.
- 组织表达：
- Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
- Western Blot analysis of various cells using p53RFP Polyclonal Antibody
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- Western Blot analysis of HeLa cells using p53RFP Polyclonal Antibody
