• 靶点：
• p53

• 简介：
• >>Endocrine resistance;>>Platinum drug resistance;>>MAPK signaling pathway;>>Sphingolipid signaling pathway;>>Cell cycle;>>p53 signaling pathway;>>Mitophagy - animal;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Ferroptosis;>>Cellular senescence;>>Wnt signaling pathway;>>Neurotrophin signaling pathway;>>Thyroid hormone signaling pathway;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Shigellosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Viral carcinogenesis;>>Proteoglycans in cancer;>>MicroRNAs in cancer;>>Colorectal cancer;>>Pancreatic cancer;>>Endometrial cancer;>>Glioma;>>Prostate cancer;>>Thyroid cancer;>>Basal cell carcinoma;>>Melanoma;>>Bladder

• 基因名称：
• TP53

• 蛋白名称：
• Cellular tumor antigen p53

• Human Gene Id：
• 7157

• Human Swiss Prot No：
• P04637

• Mouse Gene Id：
• 22059

• Mouse Swiss Prot No：
• P02340

• Rat Gene Id：
• 24842

• Rat Swiss Prot No：
• P10361

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human p53. AA range:10-59

• 特异性：
• p53 Polyclonal Antibody detects endogenous levels of p53 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• TP53;P53;Cellular tumor antigen p53;Antigen NY-CO-13;Phosphoprotein p53;Tumor suppressor p53

• 实测条带：
• 53kD

• 背景：
• tumor protein p53(TP53) Homo sapiens This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013],

• 功能：
• cofactor:Binds 1 zinc ion per subunit.,disease:Defects in TP53 are a cause of choroid plexus papilloma [MIM:260500]. Choroid plexus papilloma is a slow-growing benign tumor of the choroid plexus that often invades the leptomeninges. In children it is usually in a lateral ventricle but in adults it is more often in the fourth ventricle. Hydrocephalus is common, either from obstruction or from tumor secretion of cerebrospinal fluid. If it undergoes malignant transformation it is called a choroid plexus carcinoma. Primary choroid plexus tumors are rare and usually occur in early childhood.,disease:Defects in TP53 are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is an autosomal dominant familial cancer syndrome that in its classic form is defined by the existence of a proband affected by a sarcoma before 45 years with a first degree relative affected by any tumor before 45 years a

• 细胞定位：
• Cytoplasm . Nucleus . Nucleus, PML body . Endoplasmic reticulum . Mitochondrion matrix . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Recruited into PML bodies together with CHEK2 (PubMed:12810724). Translocates to mitochondria upon oxidative stress (PubMed:22726440). Translocates to mitochondria in response to mitomycin C treatment (PubMed:27323408). .; [Isoform 1]: Nucleus . Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4.; [Isoform 2]: Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm.; [Isoform 3]: Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells.; [Isoform 4]: Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cy

• 组织表达：
• Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.

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He-Wei granules (HWKL) combat cisplatin-induced nephrotoxicity and myelosuppression in rats by inhibiting oxidative stress, inflammatory cytokines and apoptosis. RSC Advances Rsc Adv. 2017 Mar;7(32):19794-19807 WB Rat kidney
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SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis. INTERNATIONAL JOURNAL OF ONCOLOGY 2015 Feb 01 WB Human 1:500 SKM-1 cell， K562 cell
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Targeting FoxM1 by thiostrepton inhibits growth and induces apoptosis of laryngeal squamous cell carcinoma. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 2014 Nov 13 WB Human Hep-2 cell
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CD90 affects the biological behavior and energy metabolism level of gastric cancer cells by targeting the PI3K/AKT/HIF‑1α signaling pathway. Oncology Letters Oncol Lett. 2021 Mar;21(3):1-1 WB Human 1:1000 AGS cell
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Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis. ASIAN JOURNAL OF ANDROLOGY Asian J Androl. 2020 Sep-Oct; 22(5): 493–499 WB Mouse 1: 2000 GC1 cell, GC2 cell
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DNA damage response-initiated cytokine secretion in bone marrow stromal cells promotes chemoresistance of myeloma cells. LEUKEMIA & LYMPHOMA 2017 Dec 18 WB Human BMSCs
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Effects of the combination of As2O3 and AZT on proliferation inhibition and apoptosis induction of hepatoma HepG2 cells following silencing of Egr-1. OncoTargets and Therapy Oncotargets Ther. 2018; 11: 3293–3301 WB Human HepG2 cell
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LC3-II may mediate ATR-induced mitophagy in dopaminergic neurons through SQSTM1/p62 pathway. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2018 Oct 01 WB Rat 1:1000 substantia nigra (SNpc) PC-12 cell
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Effects of 12C6+ heavy ion beam irradiation on the p53 signaling pathway in HepG2 liver cancer cells. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2017 Nov 01 WB Human HepG2 cell
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Ad-p53 enhances the sensitivity of triple-negative breast cancer MDA-MB-468 cells to the EGFR inhibitor gefitinib. ONCOLOGY REPORTS 2014 Dec 11 WB Human MDA-MB-468 cell
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UBE2W down‐regulation promotes cell apoptosis and correlates with hypospermatogenesis. ANDROLOGIA Andrologia. 2020 Feb;52(1):e13474 WB Mouse 1:2000 testis tissues
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Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and angiogenesis, and induces apoptosis of nasopharyngeal carcinoma cells. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2014; 7(9): 5450–5460 WB Human Nasopharyngeal carcinoma (NPC) cell
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Interferon-alpha responsible EPN3 regulates hepatitis B virus replication Frontiers in Medicine Guangyan Liu WB Human,Mouse
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Bufalin inhibits the proliferation of lung cancer cells by suppressing Hippo-YAP pathway. Yongchun Yu WB Human A549 cell
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Identification of the Kupffer cell-derived circulating IGFBP-3 as a universal radiation biomarker for heavy ion, proton, and X-ray exposure. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Wenjun Wei WB Mouse，Rat 1:3000 liver tissue RLSEC cell,mice Kupffer cell lines (MKC),NCTC-1469 cell
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Sunitinib induces cardiotoxicity through modulating oxidative stress and Nrf2-dependent ferroptosis in vitro and in vivo CHEMICO-BIOLOGICAL INTERACTIONS Dongjie Li WB Mouse,Rat heart tissue H9c2 cell
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POSTN promotes nucleus pulposus cell senescence and extracellular matrix metabolism via activing Wnt/β-catenin and NF-κB signal pathway in intervertebral disc degeneration CELLULAR SIGNALLING Daxue Zhu WB Human,Rat 1:1000 nucleus pulposus (NP) tissue nucleus pulposus cell
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