• 靶点：
• p15

• 简介：
• >>FoxO signaling pathway;>>Cell cycle;>>Cellular senescence;>>TGF-beta signaling pathway;>>Cushing syndrome;>>Human T-cell leukemia virus 1 infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Small cell lung cancer;>>Gastric cancer

• 基因名称：
• CDKN2B

• 蛋白名称：
• Cyclin-dependent kinase 4 inhibitor B

• Human Gene Id：
• 1030

• Human Swiss Prot No：
• P42772

• Mouse Gene Id：
• 12579

• Mouse Swiss Prot No：
• P55271

• Rat Gene Id：
• 25164

• Rat Swiss Prot No：
• P55272

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human p15 INK. AA range:89-138

• 特异性：
• p15 Polyclonal Antibody detects endogenous levels of p15 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• CDKN2B;MTS2;Cyclin-dependent kinase 4 inhibitor B;Multiple tumor suppressor 2;MTS-2;p14-INK4b;p15-INK4b;p15INK4B

• 实测条带：
• 14kD

• 背景：
• This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008],

• 功能：
• disease:Defects in CDKN2B are involved in tumor formation.,function:Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.,polymorphism:Genetic variations in CDKN2B may underlie susceptibility to uveal melanoma [MIM:155720]. Uveal melanoma is the most common type of ocular malignant tumor, consisting of overgrowth of uveal melanocytes and often preceded by a uveal nevus.,similarity:Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.,similarity:Contains 4 ANK repeats.,subunit:Heterodimer of CDKN2B with CDK4 or CDK6.,

• 细胞定位：
• Cytoplasm . Also found in the nucleus.

• 组织表达：
• Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.

Lung cancer deficient in the tumor suppressor GATA4 is sensitive to TGFBR1 inhibition. Nature Communications 2019 Apr 10 WB Human 1:1000 H460 cell, EKVX cell,Hop62 cell,A549 cell
货号：YT3492

RNA-binding protein MEX3A controls G1/S transition via regulating the RB/E2F pathway in clear cell renal cell carcinoma. Molecular Therapy-Nucleic Acids Mol Ther-Nucl Acids. 2022 Mar;27:241 WB Human ACHN cells, 293T cells
货号：YT3492

SIRT2 counteracts primate cardiac aging via deacetylation of STAT3 that silences CDKN2B. Nature Aging Guang-Hui Liu WB Mouse,Human 1:500 hearts cardiomyocytes
货号：YT3492

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