NY-CO-1 Polyclonal Antibody
- 货号:YT3215
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Nuclear export mediator factor NEMF
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human SDCG1. AA range:881-930
- 特异性:
- NY-CO-1 Polyclonal Antibody detects endogenous levels of NY-CO-1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NEMF;SDCCAG1;Nuclear export mediator factor NEMF;Antigen NY-CO-1;Serologically defined colon cancer antigen 1
- 背景:
- This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016],
- 细胞定位:
- Cytoplasm, cytosol . Nucleus .
- 组织表达:
- Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis.
- Western Blot analysis of various cells using NY-CO-1 Polyclonal Antibody
- Western Blot analysis of 3T3 cells using NY-CO-1 Polyclonal Antibody
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HeLa and Jurkat cells, using SDCG1 Antibody. The lane on the right is blocked with the synthesized peptide.