NY-CO-1 Polyclonal Antibody

    • 货号:YT3215
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • NY-CO-1
      • 基因名称:
      • NEMF
      • 蛋白名称:
      • Nuclear export mediator factor NEMF
      • Human Gene Id:
      • 9147
      • Human Swiss Prot No:
      • O60524
      • Mouse Swiss Prot No:
      • Q8CCP0
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human SDCG1. AA range:881-930
      • 特异性:
      • NY-CO-1 Polyclonal Antibody detects endogenous levels of NY-CO-1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • NEMF;SDCCAG1;Nuclear export mediator factor NEMF;Antigen NY-CO-1;Serologically defined colon cancer antigen 1
      • 实测条带:
      • 123kD
      • 背景:
      • This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016],
      • 细胞定位:
      • Cytoplasm, cytosol . Nucleus .
      • 组织表达:
      • Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis.
      • 产品图片
      • Western Blot analysis of various cells using NY-CO-1 Polyclonal Antibody
      • Western Blot analysis of 3T3 cells using NY-CO-1 Polyclonal Antibody
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from HeLa and Jurkat cells, using SDCG1 Antibody. The lane on the right is blocked with the synthesized peptide.