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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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NTF2 Polyclonal Antibody
- 货号:YT3201
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Nuclear transport factor 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human NUTF2. AA range:67-116
- 特异性:
- NTF2 Polyclonal Antibody detects endogenous levels of NTF2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NUTF2;NTF2;Nuclear transport factor 2;NTF-2;Placental protein 15;PP15
- 背景:
- This gene encodes a cytosolic factor that facilitates protein transport into the nucleus. The encoded protein is required for nuclear import of the small Ras-like GTPase, Ran which is involved in numerous cellular processes. This protein also interacts with the nuclear pore complex glycoprotein p62. [provided by RefSeq, Apr 2016],
- 功能:
- function:Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.,similarity:Contains 1 NTF2 domain.,subunit:Homodimer.,
- 细胞定位:
- Cytoplasm, cytosol . Nucleus outer membrane . Nucleus, nuclear pore complex . Nucleus inner membrane . Nucleus, nucleoplasm . At steady state it is essentially nucleoplasmic, enriched in nucleoplasmic foci. .
- Western Blot analysis of HeLa cells using NTF2 Polyclonal Antibody
- Western Blot analysis of Hela cells using NTF2 Polyclonal Antibody
- Western blot analysis of NUTF2 Antibody. The lane on the right is blocked with the NUTF2 peptide.
- Immunohistochemistryt analysis of paraffin-embedded human prostate, using NUTF2 Antibody. The lane on the right is blocked with the NUTF2 peptide.
- Western blot analysis of the lysates from HT-29 cells using NUTF2 antibody.