• 靶点：
• NOS2/iNOS

• 简介：
• >>Arginine biosynthesis;>>Arginine and proline metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>HIF-1 signaling pathway;>>Peroxisome;>>Apelin signaling pathway;>>Relaxin signaling pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Pertussis;>>Leishmaniasis;>>Chagas disease;>>Toxoplasmosis;>>Amoebiasis;>>Tuberculosis;>>Pathways in cancer;>>Small cell lung cancer

• 基因名称：
• NOS2, INOS

• 蛋白名称：
• Nitric oxide synthase inducible

• Human Gene Id：
• 4843

• Human Swiss Prot No：
• P35228

• Mouse Gene Id：
• 18126

• Mouse Swiss Prot No：
• P29477

• Rat Gene Id：
• 24599

• Rat Swiss Prot No：
• Q06518

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166

• 特异性：
• NOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IF 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• NOS2;NOS2A;Nitric oxide synthase; inducible;Hepatocyte NOS;HEP-NOS;Inducible NO synthase;Inducible NOS;iNOS;NOS type II;Peptidyl-cysteine S-nitrosylase NOS2

• 实测条带：
• 131kD

• 背景：
• Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.,function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.,induction:By endotoxins and cytokines.,online information:Nitric oxide synthase entry,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subunit:Homodimer. Bin

• 细胞定位：
• Cytoplasm, cytosol . Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. .

• 组织表达：
• Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes (PubMed:7504305).

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MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-κB pathway. Cell Death & Disease Cell Death Dis. 2021 May;12(6):1-15 WB Mouse 1 : 1000 primary murine chondrocytes，RAW264.7 cell
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PM2.5 induces autophagy-mediated cell death via NOS2 signaling in human bronchial epithelium cells. International Journal of Biological Sciences Int J Biol Sci. 2018; 14(5): 557–564 WB Human BEASE-2B cell
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Regulation of ezrin tension by S-nitrosylation mediates non-small cell lung cancer invasion and metastasis. Theranostics Theranostics. 2019; 9(9): 2555–2571 WB Human NSCLC tissue
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Zhu, Xiao-Ming, et al. "PM2. 5 induces autophagy-mediated cell death via NOS2 signaling in human bronchial epithelium cells." International journal of biological sciences 14.5 (2018): 557.
货号：YT3169

Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. CANCER SCIENCE Cancer Sci. 2020 Oct; 111(10): 3802–3812 WB Human A549 cell， H1299 cell
货号：YT3169

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Mannosylated polydopamine nanoparticles alleviate radiation- induced pulmonary fibrosis by targeting M2 macrophages and inhibiting the TGF-β1/Smad3 signaling pathway. Yuhui Hao WB Mouse lung tissue
货号：YT3169

Activation of AHR by ITE improves cardiac remodelling and function in rats after myocardial infarction. ESC Heart Failure Dajun Chai IF Rat cardiac tissue
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Uncovering the Multitarget Therapeutic Mechanism of Tong-Xie-Yao-Fang on Irritable Bowel Syndrome JOURNAL OF FOOD QUALITY Xiangxue Ma WB Rat 1:1000 colonic tissue
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The macrophage polarization in allergic responses induced by tropomyosin of Macrobrachium nipponense in cell and murine models INTERNATIONAL IMMUNOPHARMACOLOGY Yanhai Xie WB Mouse 1:1000 jejunum tissue peritoneal cell
货号：YT3169

Gut microbiota promotes macrophage M1 polarization in hepatic sinusoidal obstruction syndrome via regulating intestinal barrier function mediated by butyrate Gut Microbes Si Zhao IF,WB Mouse,Human 1:100,1:1000 liver tissue
货号：YT3169

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